Study Fails to Confirm XMRV Test Results

Konstance Knox et al. tested newly collected samples from 61 CFS patients of which 43 had Knox-study-diagram-225x300received positive test results for XMRV from either the Whittemore Peterson Institute or its commercial laboratory, VIP Diagnostics. This team used a variety of methods to detect XMRV and did not detect XMRV in any of the freshly collected samples. Following on reports from other laboratories, they tested a variety of reagents used to perform polymerase chain reaction and found 9 of 17 to be contaminated with mouse DNA similar to sequences reported by Lo et al. in the Proceedings of the National Academy of Sciences.  They conclude, “We believe that the detection of MLV in human blood in previous studies reflects contamination of reagents used to assess their presence and/or contamination of human samples during laboratory manipulation of the infectious XMRV clone, VP62.” This study attracted high profile media coverage from hundreds of outlets, in part due to the simultaneous publication of a study reporting the origin of XMRV to be a laboratory recombination of two endogenous mouse viruses and an editorial Expression of Concern by Science’s editor-in-chief about the original publication from Lombardi et al. published in Science in Oct. 2009. A more detailed analysis of this study and its conclusion is provided here on Research1st. (Science, May 31, 2011)

Tags: , May 31, 2011